Initiation of ifa5/24/2023 ![]() More importantly, ectopic expression of SDC1 was able to completely restore HCV attachment to Huh-7.5 cells in which the endogenous SDC1 expression was silenced by specific siRNAs. In contrast, the siRNA-mediated knockdown of other SDCs, GPCs, HSPG2, and agrin had no effect on HCV attachment. The silence of SDC2 expression also caused a modest decrease of HCV attachment. The knockdown of SDC1 expression by small interfering RNA (siRNA)-induced gene silence resulted in a significant reduction of HCV attachment to Huh-7.5 cells and stem cell-differentiated human hepatocytes. Substantial evidence derived from our studies demonstrates that SDC1 is the major receptor protein for HCV attachment. In the present study, we have profiled each of the HSPG core proteins in HCV attachment. ![]() The HSPG core proteins include the membrane-spanning syndecans (SDCs), the lycosylphosphatidylinositol-linked glypicans (GPCs), the basement membrane proteoglycan perlecan (HSPG2), and agrin. ![]() HS is known to covalently attach to core proteins to form heparan sulfate proteoglycans (HSPGs) on the cell surface. Our recent studies demonstrated that apolipoprotein E mediates cell attachment of hepatitis C virus (HCV) through interactions with the cell surface heparan sulfate (HS).
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